The brothers performed an acoustic set as part of Mix 104.1′s Care for Kids Radiothon.
Isaac, Zac, and Taylor also took some time out to visit patients in their rooms and also pose for pictures.
Zac, 25, recently opened up his second child, daughter Junia Rosa Ruth, who was born just before Christmas.
“[She] is such a sweet gift. It has been so much fun to get to know her and to watch her along with her big brother [Shepherd] grow and change so quickly,” he told People.